Geologists
like me teach students and conduct research with microscopes called
"polarizing" or "pol" for short. After development in the late 19th century,
pol scopes became critical tools for identifying the minerals and structures
of rocks (our Earth), and for determining much about how they formed. Such users are
petrologists, who are doing petrography. This type of microscope is also used by
mineralogists, geophysicists, soil scientists, crystal chemists, materials engineers, medical researchers,
asbestos consultants, and many others including biologists. The method that can be
employed with these instruments is called PLM (polarized light microscopy).
"Simple polarizing" scopes are usually just biological microscopes with pol
filters, one inserted under the head and another in a substage filter
holder. Any scope can be so modified, including stereo microscopes (but the
common style is a compound microscope). Those designed for geological
work are also known as "petrographic" (="rock imaging") microscopes, which have accessories to assist mineral studies
in rock sections.
Many crystalline materials (organic as well as
inorganic) produce interesting optical effects with both polarizers in
place, and some biological samples can show useful changes of contrast
against a dark background.
In my research lab, an Olympus BH2 BHSP petrographic
polarizing microscope (below)
is serving me well, and I am convinced that it is the best of the 20th
century "benchtop" pol microscopes. I will use it to illustrate
pol scopes.

You might disagree with this assessment, but in my research and business I have
used more than two dozen different petrographic microscopes of many brands and models. Actually, I
liked them all, but some were better than others in design, function, and
quality.
Six of the best
pol scopes in my experience are the subject of
my article in
the internet journal called
Micscape, also linked below.
I have also described some interesting
student petrographic microscopes in
that journal. More recently, I have added a super-wide head and eyepieces
with a view field number of 26.5. It includes about 2.65 mm of slide in the
view with the 10x objective, or 6.625 mm with the 4x, which is huge. Very
nice for scanning coarse grained rock slides. But I had to renovate stuck
diopters on the oculars for it, which I described in
this Micscape article.
Another Micscape article
describes the
path to obtaining my BHSP, including earlier petrographic microscopes
that I used for geological research. New 21st century models such as
the Zeiss Axioskop Pol or Olympus BX-51P are no doubt even better
microscopes than my late 1980s machine, but it will be a long time before
such new and very expensive microscopes come into my budget range. In fact,
there are a good number of late 20th century microscopes that can meet
personal and professional requirements, and also be satisfying to own and
use.
How is it different?
There is nothing especially mysterious or strange
about the polarized light accessories used in a petrographic microscope. Its
brightfield capabilities remain the same as for any good biological
microscope, but with extra parts that can be used or not used, as you wish.
The polarizing filters are placed above and below the sample, and the upper
one can be easily moved in and out of the light path. A circular stage rotates to show how different orientations of
the sample affect polarized light, but it can also be fixed in place for
other uses. A Bertrand lens (sometimes only a pinhole with a magnifier) is
available to observe polarized light patterns on the back of a higher-power
objective. A slot allows the insertion of a filter that adds or subtracts
portions of wavelengths of light, called a compensator or wave plate. In
models designed after the 1970s, the parts for PLM functions are commonly in
modular pieces that can be easily added (or removed) on a standard
brightfield microscope stand.
Polarized Light (PLM)
So, what happens to light in a pol scope? We
can think of light as moving in linear rays, with photons vibrating
in a plane along the ray, like waves on a shaking rope. Different wave
lengths within these vibration planes appear as different colors. Raw light
is comprised of many wave lengths and vibration orientations, but a
polarizing filter squeezes light into a single plane. Essentially the filter
allows through the portions of light that can be oriented into its
vibration plane, and blocks the light that cannot fit that direction. The
second filter (called the analyzer) has its vibration plane oriented
perpendicular to the first, and so no light will pass through it. That is, no light
if only air, liquid, glass, or other isotropic material is present between the filters.
Minerals also polarize light, due to the
layers or planes of atoms in their crystal structures. Most actually split
and polarize light into two different vibration planes (two refractions, or
birefringent). So, if you place a
birefringent mineral between the two pol filters, the polarized light coming to it from
the first filter is converted into one or two new directions of vibration
planes, and some of that light can get through the second filter to reach
your eye. Only part of the original light makes it all the way, thus
the need for a strong light source.
Light slows down as it moves into the mineral,
and the two new polarizing vibration planes conduct light at different rates
in the mineral. Each light ray also bends (refracts) as it passes in and out
of the mineral, with an angle proportional to its change in velocity. Then they accelerate back to the same speed in the air out
the other side. However, the light waves of the rays are
now out-of-sync because they moved at different velocities within the
mineral. When the wave planes are combined into one plane by the second
filter, the waves "interfere" with each other, producing new wavelengths
with startling new "interference colors." This amazing feature of
birefringence (two refractions) is caused by the two mineral light rays.
Birefringence and interference colors vary according to the particular
crystal structure unique to each mineral, and so we can interpret important crystallographic properties
and identify microscopic minerals, and often infer some of their chemical compositions.
Tools such as compensator wave plates can also
provide clues to identification, while reference books provide data tables,
optical descriptions, and methodology. There are many written for students
and professionals, and you should have one or more. If the book is a few years or decades old, not a problem, as the technique has not
changed much, and the minerals not at all! My own library is rather dated,
actually. Phillips has several chapters on the use of the universal stage,
while Kerr has a fine section on mineral characteristics. Ehlers is one of
the most comprehensive resources, and petrography/atlas books are
great for illustrating what you actually see. These favorites are:
Optical Mineralogy by Paul Francis Kerr (1977, McGraw Hill)
Optical Mineralogy by Ernest Ehlers (in two volumes, 1987, Blackwell
Scientific)
Mineral Optics by Wm. Revell
Phillips (1971, Freeman)
Petrography of Igneous and Metamorphic Rocks by A. R. Philpotts (2003,
Prentiss Hall)
Atlas of Rock-Forming Minerals in Thin Section by Mackenzie and Guilford
(1984, Longman)
Any crystalline material might reveal features
of its structure and chemistry via PLM, including wonderful new colors.
Not just inorganic rocks and minerals, but also hard parts of
plants and animals, and organic crystals formed by diseases. Interesting and
beautiful effects might appear as well as new details, so it is worth a look for almost any
sample you are studying. And that is easy to do with a pol scope.
A
few images from my basalt slides are below, showing
interference colors typical of common birefringent minerals.
See
this
Idaho State University page
about thin sections of rocks, which since the late 1800s have
provided the evidence for much of what we know about the Earth. Where would science be without
microscopes?

Although all the major makers produce models of polarizing
microscopes (see below), there has been some decline during recent years in
their use in geological education and research. Considering that the Earth
is made of rocks, and rocks cannot be studied or understood without
petrographic microscopes, this is not good! See this
article by Mickey Gunter about the
problem.
Here is a separate page about Leitz Wetzlar
microscopes, including photos, parts, accessories, documents, and
links. All types, not just pol scopes. Other brands (including Leica) will
stay on this page for now. These are files that I have collected or borrowed
and saw a need to organize. There is not much before the large models of the
1960s and later, so please
send me corrections and additions
of documents to help the page grow. Model names, variations, and
options for Leitz microscopes are complex and confusing -- it would be
nice if someone (not me) started a Leitz microscopes Yahoo Group or website.
Here is a separate page about Olympus microscopes,
especially the BH2 line, including photos, links, and documents. Biological
too, not just pol types. I have a lot of documents and photos of my favorite
models from Olympus. This page is mainly about the CH, CH2, BH, and BH2
models, plus some older ones, but I will leave the newer BX and other lines
for a future effort.
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Several important
features common to petrographic microscopes are illustrated in this photo of
the middle section of my Olympus BH2 pol scope. Between the head and the
arm is an intermediate tube with a Bertrand lens, an analyzer filter, and
6x20 mm slot for a compensating wave plate. The Bertrand lens has a
dial to swing it in and out of the light path, with a second dial to focus
it. The analyzer (upper polarizing filter) can be moved in and out via a sliding
plate, and its part of the tube is marked in degrees for rotation, with a
locking screw. Wave plates for the open slot have notches to allow precise
stops for an open hole or the wave filter.
The nose turret slides on a
dovetail for easy replacement, and holds four DPlan objectives (here 4x,
10x, 20x, 40x) designated PO for their strain-free construction. Three of
the turret holes can be centered via side screws, using special small
wrenches, or because these are easily lost and hard to replace, small
screwdrivers. The same wrenches fit two centering screws for the circular
stage, which is graduated in degrees with a lever to engage a "click" every
45 degrees, and a stop screw to lock the stage in place. A special x-y
mechanical stage (slide holder) is made with low control knobs, so as to not
interfere with the objectives during rotation. It is removable because some
petrographers prefer to move slides by hand.

Beneath the stage is a Pol (strain-free) condenser
with a flip-up top lens, n.a. 0.25/0.90. This top lens concentrates light for the
higher-power objectives, needed when the Bertrand lens is used to make a
"conoscopic" view of an "interference figure" (a diagnostic light pattern
formed on the the back lens surface of the objective). The lower part of the
condenser holds a polarizing filter that can be rotated, with a click for
90-degree "crossed polarizers." On my Olympus, all of these special pieces are
very
well made, and I take good care of them because they are expensive!
Glass can become slightly polarizing under uneven
pressure or if it is not cooled evenly, leaving residual "strain."
Because there is usually a pol filter in the light path (under the condenser), an extra source of polarization will add unwanted birefringent effects,
so optical parts
are constructed (or carefully selected from normal production) to minimize
strain. Such objectives, eyepieces, and condensers might be marked P, Po, Pol or SF
(strain free), or have red lettering. However, many "regular" optical parts
might work OK in your pol scope as well, so it is worth a test. A bigger
problem is reflection polarization by mirrors and prisms in binocular and trinocular
heads, which is why many older pol scopes use monocular heads. Two-eye
viewing is worth some extra cost if you can find a binocular pol head, or if
not, you might be willing to put up with slight induced birefringence from a
standard biological head. Of course, there will be none if you can swing out
or remove both of the pol filters when viewing in "plain" light.
Because they were made in relatively small numbers, or
their modular parts could be rather specialized, petrographic microscopes
both new and used command premium prices -- often from double to triple the
price for a biological version of the same model, depending on how complete
it is. If you are outfitting one with additional optical parts, you might
find that less expensive objectives, eyepieces, and even condensers from
biological (i.e. not certified as strain free) versions work just fine, or
at least are so close that their strain effects are hardly noticeable. But
if you are buying one at its higher petrographic price, it should already
have the proper strain-free versions of optics to get your money's worth
(and to preserve its higher resale value).
For several years I counted items with
"microscope" in their titles as listed on eBay.com, by brand and type. See
eBay Microscopy (an excel spreadsheet) for
a table and charts of changes by month in these numbers. A high
total near 10,000 items was reached in August 2009, but as the recession eased,
the number of microscope listings dropped. Prices dropped too, but have
since come back (most are still bargains, but watch out). In 2010, I stopped counting because eBay changed the way it lists items in my microscope categories,
and the new counts can not be compared with my record. As
you might guess, the big four are the most popular brands, but there are
plenty of AO and B&L scopes and parts out there as well. eBay prices for
microscopes remain at bargain levels, but there are now a lot more sold at
fixed prices, whereas auctions of valuable items at low starting prices are
no longer so common.
Also see these notes about
adapting Nikon digital cameras
to my Olympus BH-2 microscope.
On
eBay are my Guides to
Buying Microscopes
and
Caring for Microscopes. If you find them to
be useful, please check the response boxes so I can stay in the top 1000!

Below are galleries of polarizing microscopes both
new and old, some interesting accessories, links to useful websites, and
documents including pdf files of scanned instruction manuals.
Please consider sharing your pdf documents and jpeg
images related to polarizing microscopes (email
to me). Thanks to these fine folks for permission to use
their images and documents:
American Optical Spencer and Polarstar
manual -- William Gasser
AO-Spencer documents on
Steve Neeley's website
AO Pol Scopes Catalog --
Humboldt St. Univ. Scientific Instr. Museum
AO Spencer Pol #41 -- Jay Stanley of
Classic Optics
B&L Dynoptic Pol scope -- Frank Nasser @
microlites.com
Cooke polarizing microscopes brochure -- Mark Glusker
Gillett & Sibert student scope -- Richard Morris (bluecerise400)
Science-Info website for
microscope documents
-- Gordon Couger
Swift and Son lab scope, and Wild M21 objectives -- Ray Sloss
Leitz Laborlux and SM Lux Pol scopes --
Ridge Equipment
Leitz Ortholux Pol scope and pol parts -- Tamagno
Leitz Ortholux camera and light meter -- William
Day
Leitz Aristomet research scope -- Chip Sanders (mr-keyboard)
Leitz AM Pol and SM Pol scopes --
Raymond Hummelink
Leitz MOP image and 1913 catalog -- Charla Mason
Leitz HM Lux pol scope and brochures -- Allen
Carpenter
Leitz older documents -- Don Grybeck
Lomo Min-8 benchtop scope -- Dirk Marel (eapoecistron)
Microscope documents from
Gordon Couger's website
Nikon Ske pol scope -- Jay Stanley of
Classic Optics
Nikon Alphaphot2 Pol -- John Woodhouse
Nikon Optiphot Pol manual -- Ian Hutcheon
Nikon Microphot Pol image --
petunia_d (eBay
seller)
Olympus POM and accessories -- Mike Symons
Olympus AH/BH/CH pol scopes brochure --
Jay Stanley of
Classic Optics
Olympus Vanox -- Mark Home (microscopesolutions)
Reichert Zetopan --
electrowise
Unitron brochure -- Jason
Weinstein
Zeiss Pol brochures and pricelist -- Paul
V. Heinrich
Zeiss Ultraphot II scope -- Frank Nasser @
microlites.com
Zeiss and Leitz price list catalogs -- Jason
Weinstein
Zeiss aus Jena benchtop scope --
BIGGERBY2002
About documents and images posted
and linked below: Many are scans that I have made from my personal
collection of old manuals and brochures, or are my photos of microscopes I
have owned. Many others are posted by special permission but remain
the property of the generous donors. Images, documents, and web files might
have no copyright notices, but that does NOT mean they are public property,
as any corporate lawyer could tell you. Some unscrupulous vendors on eBay
use and sell scans without permission, but the ones here are for personal
reference only, not for sale or commercial use. If you or your company own rights to any of the files
below, and you object to having them posted here, please
let me know and I
will remove them or their links immediately.
Documents,
files, links, and images on these pages
are for your personal use and information, and many are my property or the
property of people who have allowed me to post them here. They are
not for
commercial use including eBay
and may not be altered, sold, distributed, or otherwise used without specific permission from me
or the people who own them.
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